Purigen Biosystems, Inc.

PURIGEN ISOTACHOPHORESIS

The Revolutionary Nucleic Acid Purification Technology
Move beyond 20+ year-old DNA extraction methods
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The Problem

 

Column, bead, and precipitation-based kits used for extracting nucleic acids all follow the same fundamental workflow.

Traditional DNA Extraction Method

Cellular lipid membranes are broken down through the use of detergents, releasing proteins and nucleic acids into a solution. Proteases cleave proteins and strong salts, termed chaotropic salts, will further denature them along with dehydrating molecules.

The Solution

 

Charge is a fundamental attribute of any biological molecule; in solution, it can be used not only to separate molecules, but also to concentrate and focus them.

Purigen Charged-basee DNA Extraction

Standard separation techniques commonly rely on multiple analyte properties, such as differences in charge, size, solubility, and/or binding affinity, in addition to a solid surface. Isotachophoresis separates and focuses charged molecules in solution solely based on their ionic mobility.

A Superior Approach to Nucleic Acid Purification

Isotachophoresis separates and concentrates charged molecules in solution solely based on their electrophoretic mobility.
Biological samples are gently lysed and added to the Purigen Ionic Fluidics Chip. An electric field is then applied to the chip and
the nucleic acid is isolated in its natural, native form. The nucleic acid is not denatured or dehydrated, and there’s no binding to,
or stripping from, fixed surfaces. The result is a higher yield of pure nucleic acid that is less fragmented and free from
bead or wash buffer contamination.

Simple Charge-based Sample Prep in Solution

Purigen Charge-based Sample Prep