WHITE PAPER

Isotachophoresis Improves Nucleic Acid Extraction and Purification from FFPE Samples

OVERVIEW: Researchers and clinicians working with FFPE tissue samples have become accustomed to managing labor-intensive workflows to extract and purify nucleic acids for molecular analyses. Such workflows have the potential to further damage nucleic acids during extraction and introduce bias to downstream analyses. As this white paper discusses, the application of isotachophoresis to purify nucleic acids without using an intermediary binding surface yields higher quality nucleic acid and several benefits to downstream analyses. The white paper reviews a series of experiments conducted with the Purigen Biosystems Ionic™ Purification System to demonstrate an automated application of isotachophoresis and the resulting improvements to workflow, nucleic acid yield, and nucleic acid quality by comparison to conventional methods with data from qPCR-based assays and next-generation sequencing.

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