Ionic® Purification System
Nucleic Acid Purification – Pure and Simple®REQUEST QUOTE VIEW BROCHURE
The Ionic Purification System is so different, its advantages are most readily understood in contrast with conventional DNA and RNA extraction and purification methods.
The Ionic Purification System uses isotachophoresis (ITP)
to extract, purify, and concentrate genomic DNA and RNA
from cells and FFPE. DNA and RNA samples prepared
by the system are pure, abundant, and ready in just 60 minutes.
Purigen’s simplified workflow minimizes opportunities for material loss and errors. In a single automated step, the Ionic system yields pure, high-concentration DNA and RNA in a low-salt buffer.
qPCR results from the amplification of a 280bp amplicon in DNA purified from cell populations ranging from 1,120 to 56,000 cells demonstrate that purification on the Ionic system results in values that are ~3 Ct lower than those from a column-based method. For populations with fewer cells, some eluates from the column-based competitor do not contain detectable amounts of DNA.
Results for 32 FFPE block samples* purified from scrolls for Purigen (no paraffin removal) vs. slides (excess paraffin removed) for a column-based approach. Yields for Purigen samples demonstrate improved amplifiable yields of on average 3.5x versus column-based approach.
NGS results of DNA purified from FFPE samples analyzed using the Agilent SureMASTR Tumor Hotspot sequencing panel with 252 amplicons ranging from 128 bps to 245 bps show that samples purified on the Purigen Ionic system have tighter clustering when compared to samples prepared using a column-based method – indicative of more uniform sequence coverage.
Taking a closer look at the results from above shows that DNA from FFPE samples purified on the Ionic system is less biased toward GC content and fragment length than from FFPE samples purified using a column-based method.
*Data for the graphs above is normalized by a reference sample to remove the effect of coverage differences introduced by the amplification efficiency of the target amplicon. Data from the reference sample incorporates purification results from the Purigen system and a column-based method to avoid biasing the reference sample toward either technology.
Dimensions and Weight
|Height||42.4 cm / 16.7 in|
|Width||41.9 cm / 16.5 in|
|Depth||48.9 cm / 19.3 in|
|Weight||24.5 kg / 54 lb|
|North America / UL||120 VAC, 60Hz, 2.5A|
|Europe / CE||230 V~50 Hz, 1.0A|
|Power consumption||300 watts|
|North America / UL||• 61010
• CFR title 47, sub part B of part 15 / ICES-003, Issue 6
|Europe / CE||• EN61010-1:2010
• EN 61326-1-2013
• WEEE Directive 76/769/EC
Isotachophoresis separates and concentrates charged molecules in solution solely based on their electrophoretic mobility. Biological samples are gently lysed and added to the Purigen Ionic® Fluidic Chip which applies an electric field to isolate nucleic acid in its natural, native form.