Purigen Biosystems, Inc.

A Superior Approach to Nucleic Acid Extraction

The Ionic Purification System is so different, its advantages are most readily understood in contrast with conventional DNA extraction and purification methods.


  • No organic solvents
  • No harsh, high-salt buffers
  • No system programming
  • No beads or repetitive washing
  • No hands-on mixing, separation, sample transfers, or buffer exchanges
  • No pumps, valves, or other moving parts

Ionic Purification System

The Ionic Purification System from Purigen Biosystems uses
isotachophoresis (ITP) to extract, purify, and concentrate
genomic DNA
from cells and and FFPE. DNA samples prepared
by the system are pure, abundant, and ready in just 60 minutes.

 

Easy to Use and Small Footprint

Pure Nucleic Acid in Just ONE Hour

Purigen’s simplified workflow minimizes opportunities for material loss and errors. In a single step, the Purigen Ionic system yields pure,
high-concentration DNA in a low-salt buffer.

  1. 1 Load Run Buffers

    Add buffers from reagent kit –
    use touchscreen to start priming

    8 mins

  2. 2 Load Sample

    Load 8 samples –
    use touchscreen to start the run

    50 mins

  3. 3 Get Purified Nucleic Acid

    Collect samples when touchscreen displays
    “Run Complete”

    2 mins

Purification in just 3 minutes of hands-on time per sample

Higher Amplifiable Yield

Extract Up to 50% More Nucleic Acid from Cells and FFPE

 

qPCR results from the amplification of a 280bp amplicon in DNA purified from cell populations ranging from 1,120 to 56,000 cells demonstrate that purification on the Ionic system results in values that are ~3 Ct lower than those from a column-based method. For populations with fewer cells, some eluates from the column-based competitor do not contain detectable amounts of DNA.

 

Achieve Yields of 3.5x or More from FFPE Samples

Purigen SCROLLS vs. Column-based Kit SLIDES

 

Results for 32 FFPE block samples* purified from scrolls for Purigen (no paraffin removal) vs. slides (excess paraffin removed) for a column-based approach. Yields for Purigen samples demonstrate improved amplifiable yields of on average 3.5x versus column-based approach.

 


Higher Quality Results

More Uniform Coverage

 

NGS results of DNA purified from FFPE samples analyzed using the Agilent SureMASTR Tumor Hotspot sequencing panel with 252 amplicons ranging from 128 bps to 245 bps show that samples purified on the Purigen Ionic system have tighter clustering when compared to samples prepared using a column-based method – indicative of more uniform sequence coverage.

 

No Bias Toward Length or GC Content of Amplicons

 

 

Taking a closer look at the results from above shows that DNA from FFPE samples purified on the Ionic system is less biased toward GC content and fragment length than from FFPE samples purified using a column-based method.

 

*Data for the graphs above is normalized by a reference sample to remove the effect of coverage differences introduced by the amplification efficiency of the target amplicon. Data from the reference sample incorporates purification results from the Purigen system and a column-based method to avoid biasing the reference sample toward either technology.

 

System Specifications

Dimensions and Weight (with leveling platform)

Height44 cm / 17.3 in
Width45 cm / 17.7 in
Depth52 cm / 20.5 in
Weight24.5 kg / 54 lb

Facility Requirements

Power110-120 VAC, 60Hz, 4A

Regulatory Compliance

UL61010
FCCCFR title 47, sub part B of part 15 / ICES-003, Issue 6