Purigen Biosystems, Inc.

Ionic Kits by Application

Ionic Purification Kits and protocols support purfication of specific sample types and are required for use with the Ionic Purification System. Purification protocols are developed and validated at Purigen Biosystems to maximize purification yield and quality with minimal manual steps.

Ionic® Cells to Pure DNA Kit
Ionic Cells to Pure DNA Kit

Each Cells to Pure DNA Kit contains 6 Purigen Ionic Fluidic Chips and reagents to purify DNA from 48 samples.


Sample

Sample type Cultured cells
Sample preps per kit 48
Sample preps per chip 8

Density

Maximum cell density per sample 100,000 cells (diploid equivalents)
Minimum cell density per sample 1,000 cells (diploid equivalents)

Volume

Input volume per sample prep ~210 µL of lysate
Elution volume per sample prep ~40 µL

Run-time

Run-time per chip ~60 mins

Contents

Fluidic Chip 6 qty
Lysis Buffer 1 6 bottles; 1.2 mL
Lysis Buffer 2 1 bottle; 7.2 mL
RNAse Reagent 1 bottle; 100.0 µL
Proteinase K Reagent 1 bottle; 150.0 µL
Extraction Buffer 1 bottle; 12.0 mL
Anodic Buffer 1 bottle; 12.0 mL
Separation Buffer 1 bottle; 18.0 mL
Neutralization Buffer 1 bottle; 12.0 mL
Cathodic Buffer 1 bottle; 12.0 mL

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Ionic® FFPE to Pure DNA Kit
Ionic FFPE to Pure DNA Kit

Each FFPE to Pure DNA Kit contains 6 Purigen Ionic Fluidic Chips reagents to purify DNA from 48 samples.


Sample

Sample type FFPE scroll / tissue section
Sample preps per kit 48
Sample preps per chip 8

Cross-section

Recommended cross-sectional area per sample 50 mm2 – 300 mm2

Volume

Input volume per sample prep ~200 µL of lysate
Elution volume per sample prep ~40 µL

Run-time

Run-time per chip ~60 mins

Contents

Fluidic Chip 6 qty
Lysis Buffer 6 bottles; 1.8 mL
DNAse Reagent 1 bottle; 250.0 µL
Proteinase K Reagent 1 bottle; 480 µL
Extraction Buffer 1 bottle; 12.0 mL
Anodic Buffer 1 bottle; 12.0 mL
Separation Buffer 6 bottles; 2.5 mL
Neutralization Buffer 1 bottle; 12.0 mL
Cathodic Buffer 1 bottle; 12.0 mL
Mineral Oil 1 bottle; 15.0 mL

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Ionic® FFPE to Pure RNA Kit
Ionic FFPE to Pure RNA Kit

Each FFPE to Pure RNA Kit contains 6 Purigen Ionic Fluidic Chips and reagents to purify RNA from 48 samples.


Sample

Sample type FFPE scroll / tissue section
Sample preps per kit 48
Sample preps per chip 8

Cross-section

Recommended cross-sectional area per sample 50 mm2 – 300 mm2

Volume

Input volume per sample prep ~200 µL of lysate
Elution volume per sample prep ~40 µL

Run-time

Run-time per chip ~60 mins

Contents

Fluidic Chip 6 qty
Lysis Buffer 1 6 bottles; 1.4 mL
Lysis Buffer 2 1 bottle; 4.0 mL
DNAse I Reagent 1 bottle; 600.0 µL
Proteinase K Reagent 1 bottle; 1.0 mL
Extraction Buffer 1 bottle; 12.0 mL
Anodic Buffer 1 bottle; 12.0 mL
Separation Buffer 6 bottles; 2.5 mL
Neutralization Buffer 1 bottle; 12.0 mL
Cathodic Buffer 1 bottle; 12.0 mL
Mineral Oil 1 bottle; 20.0 mL

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Ionic Reagents and Kit

 

 

Purigen Isotachophoresis Technology

Isotachophoresis separates and concentrates charged molecules in solution solely based on their electrophoretic mobility. Biological samples are gently lysed and added to the Purigen Ionic® Fluidic Chip which applies an electric field to isolate nucleic acid in its natural, native form.