Fragoso joined the cancer program after completing her Ph.D. in pathology at Sao Paulo State University in Brazil. She studies microRNA expression in ulcerative colitis-associated colorectal cancer cases. In her experiments, Fragoso works with preclinical mouse models, in vitro cancer cell lines, and patient tumor biopsies.
For these studies, Fox Chase scientists work with microRNA expression data and RNA sequencing results, as well as protein information from immunohistochemistry and ELISA experiments. In one project, Fragoso analyzed formalin-fixed, paraffin-embedded (FFPE) biopsies collected from patients at various stages of ulcerative colitis. She used the Purigen Ionic® Purification System and the Ionic® FFPE to Pure RNA kits to extract RNA from her samples.
The goal of this study was to identify a biomarker signature associated with the development of precancerous lesions in patients with ulcerative colitis. Over time, patients with this disease can develop two kinds of dysplasia: one with a flat morphology and one with an elevated growth component (mushroom-like). Although these high-risk individuals routinely undergo colonoscopies to identify tumors early in their development, flat lesions are much harder to detect and can be missed until it is too late.
Most scientists are familiar with the challenges of extracting RNA from FFPE samples. Besides low yields, such RNA is often contaminated or badly degraded, making downstream analysis difficult. In earlier projects, Fragoso frequently found herself struggling to get sufficient RNA for analysis from biopsies. And there was very little room for error given the amount of sample available.
There were also a lot of steps involved in preparing the samples and scientists had to monitor the column during the experiment.
These are exactly the kinds of situations that the Purigen system and kits were designed to address. Fragoso was introduced to the Purigen technology by her lab supervisor. She tested the system on a handful of samples to see how it stacked up against the existing column-based method in the lab.
The difference between the two approaches was immediately clear, from the sample prep through to the final output.
It also has fewer steps than the column-based approach. For example, there is no need for a deparaffinization step; the Purigen system takes care of this during the run. Once samples are loaded, scientists can work on other tasks while the machine runs.
The yield is also significantly better. From a 10-micron FFPE sample, Fragoso was able to extract about 17 nanograms of RNA per microliter using the Purigen system.
Access to sufficient RNA from FFPE samples is enabling Fragoso to make discoveries that could improve cancer detection and prevention in the future. In one study, she extracted RNA from biopsy samples from ulcerative colitis patients and analyzed microRNA expression. She successfully validated one of the microRNAs — miR-200a — in the proposed cancer detection signature. She also constructed a network of the genes targeted by all of the microRNAs in the signature and confirmed a prior prediction that when miR-200a is upregulated, it downregulates the expression of a gene that regulates methylation. Fragoso and her colleagues are now trying to understand the role of this interaction in colorectal cancer development among ulcerative colitis patients.
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