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Ionic® Cells to Pure DNA
Higher yields of high quality DNA from up to 5 million cells
VIEW BROCHURE GET QUOTEThe Cells to Pure DNA Kit supports a wide range of cell types including WBCs or PBMCs isolated from blood as well as cultured or sorted cells. The standard input range is 50k to 5 million cells. Customized protocols for as few as 10 cells are available upon request.
The Ionic Purification System from Purigen Biosystems uses isotachophoresis (ITP) to extract, purify, and concentrate genomic DNA from cells and and FFPE. Since nucleic acids remain in their native form, not denatured or dehydrated, the Ionic system is ideal for challenging samples with limited starting material or low-quality material.
FIGURE 1: Peripheral Blood Mononuclear Cells (PBMCs) were isolated from whole blood via a ficoll gradient, quantified, and processed on the Ionic Purification System and a market-leading column-based kit in quantities of 1 to 5 million cells. DNA extracts were then quantified via Qubit (Concentration and Yield) and PCR (Amplifiable Yield). The Ionic DNA kit extracts had yields that were 2x to 2.5x those observed with the column-based kit
FIGURE 2: White Blood Cells (WBCs) were isolated from whole blood by lysing the red blood cells (RBCs) and pelleting the WBCs. The pellet was washed, quantified, and processed on the Ionic Purification System and a market-leading column-based kit in quantities of 1 to 5 million cells. DNA extracts were then quantified via Qubit (Concentration and Yield) and PCR (Amplifiable Yield). The Ionic DNA kit had yields that were 3x to 6.5x those observed with the column-based kit.
FIGURE 3: DNA Extracts from the Ionic System were evaluated on a Tapestation to determine their size profile. Size profiles were consistent across cell type and quantity.
Isotachophoresis separates and concentrates charged molecules in solution solely based on their electrophoretic mobility. Biological samples are gently lysed and added to the Purigen Ionic® Fluidic Chip which applies an electric field to isolate nucleic acid in its natural, native form.
