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Purigen Biosystems, Inc.

Ionic® Cells to Pure DNA

Maximum Recovery from Blood-based or Cultured Cells

Higher yields of high quality DNA from up to 5 million cells

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Maximize Yields For WBCs, PBMCs, and Cultured/Sorted Cells

The Cells to Pure DNA Kit supports a wide range of cell types including WBCs or PBMCs isolated from blood as well as cultured or sorted cells. The standard input range is 50k to 5 million cells. Customized protocols for as few as 10 cells are available upon request.


  • NEW High-Input Protocol – Enables extraction from 50,000 to 5 million cells
  • Optimized for White Blood Cells – Higher concentration and yields from WBCs and PBMCs
  • Wide Range of Inputs for Cultured/Sorted Cells – Utilize standard protocol above 50K cells or enquire about optional low-input protocols supporting as few as 10 cells

Ionic® Purification System for Cells

The Ionic Purification System from Purigen Biosystems uses isotachophoresis (ITP) to extract, purify, and concentrate genomic DNA from cells and and FFPE. Since nucleic acids remain in their native form, not denatured or dehydrated, the Ionic system is ideal for challenging samples with limited starting material or low-quality material.


Significantly Higher Concentration and Yield from PBMCs


 

FIGURE 1: Peripheral Blood Mononuclear Cells (PBMCs) were isolated from whole blood via a ficoll gradient, quantified, and processed on the Ionic Purification System and a market-leading column-based kit in quantities of 1 to 5 million cells. DNA extracts were then quantified via Qubit (Concentration and Yield) and PCR (Amplifiable Yield). The Ionic DNA kit extracts had yields that were 2x to 2.5x those observed with the column-based kit

Significantly Higher Concentration and Yield WBCs


 

FIGURE 2: White Blood Cells (WBCs) were isolated from whole blood by lysing the red blood cells (RBCs) and pelleting the WBCs. The pellet was washed, quantified, and processed on the Ionic Purification System and a market-leading column-based kit in quantities of 1 to 5 million cells. DNA extracts were then quantified via Qubit (Concentration and Yield) and PCR (Amplifiable Yield). The Ionic DNA kit had yields that were 3x to 6.5x those observed with the column-based kit.


 

Consistent Length Profiles With Average Length Above 20 Kbp


 

FIGURE 3: DNA Extracts from the Ionic System were evaluated on a Tapestation to determine their size profile. Size profiles were consistent across cell type and quantity.

 

Purigen Isotachophoresis Technology

Isotachophoresis separates and concentrates charged molecules in solution solely based on their electrophoretic mobility. Biological samples are gently lysed and added to the Purigen Ionic® Fluidic Chip which applies an electric field to isolate nucleic acid in its natural, native form.