Ionic® Cells to Pure DNA - Low Input
Higher yields of high quality DNA from a few as 10 cells
DOWNLOAD BROCHURE GET QUOTEThe Ionic system consistently recovers DNA in yields near the theoretical maximum from cultured or sorted cell samples in amounts ranging from 100,000 cells down to just 10 cells. Regardless of the input amount, the workflow for the Ionic Cells to Pure DNA – Low Input Kit is the same and does not require the use of carrier RNA.
The Ionic Purification System from Purigen Biosystems uses isotachophoresis (ITP) to extract, purify, and concentrate genomic DNA from cells and and FFPE. Since nucleic acids remain in their native form, not denatured or dehydrated, the Ionic system is ideal for challenging samples with limited starting material or low-quality material.
FIGURE 1: DNA extracted and purified from 3 cell lines at input amounts of 10, 100, 1000, 10,000 and 100,000 cells using the Ionic system and a commercially available column based kit. Ionic system yields increase linearly as input amount increases and range from 1.5x to 2x those of the column kit. Yield of DNA recovered on the Ionic system is 80-90% of theoretical maximum yield.
FIGURE 2: 12 cell samples purified using Purigen and a conventional column-based approach (replicates of 6 for each technology). Samples purified with the Ionic system show clean target bands; without smearing and less fragmentation.
Isotachophoresis separates and concentrates charged molecules in solution solely based on their electrophoretic mobility. Biological samples are gently lysed and added to the Purigen Ionic® Fluidic Chip which applies an electric field to isolate nucleic acid in its natural, native form.
